User:SuniGhimire/sandbox

Genome profiling

Genome profiling

Genome profiling (GP) is a biotechnology that acquires genome information without sequencing. It can be used for identification and classification of organisms. It was pioneered by Japanese biophysicist Prof. Koichi Nishigaki and his colleagues at Saitama University in 1990 and later<ref>https://doi.org/10.1246/cl.1991.1097</ref>. In GP, small fragments of genomic DNA are randomly amplified (random PCR) and the random PCR products are subjected to temperature-gradient gel electrophoresis (TGGE) to generate a species-specific mobility pattern (genome profile). From this, species identification dots (spiddos) are assigned<ref>https://doi.org/10.1093/nar/30.10.e42</ref>. This approach is clearly superior because it does not require prior knowledge of any gene sequence. It is clear that random PCR can produce commonly conserved genetic fragments (ccgf), which make it possible to measure the difference between organisms <ref>https://doi.org/10.1093/nar/30.10.e42</ref>. The GP method has been successfully applied to a wide range of organisms, from viruses and bacteria to animals and plants, for identification and classification <ref>https://doi.org/10.1093/bfgp/elae047</ref>. Its unique merit is in the ultra-high performance to obtain the final results (identification and classification), since GP, in principle, requires only a single random PCR plus μTGGE experiment (~2 h task in all)

Genome profiling (GP) is a biotechnology that acquires genome information without sequencing. It can be used for identification and classification of organisms. It was pioneered by Japanese biophysicist Prof. Koichi Nishigaki and his colleagues at Saitama University in 1990 and later<ref> Nishigaki, K., Amano, N., Takasawa, T., Kinoshita, Y. Husimi, Y. DNA profiling—method and principle. Seibutsubutsuri (Bull. Biophys. Soc. Jpn.), 1990; 30: S230, .ja / Nishigaki, K., Amano, N. Takasawa, T. DNA profiling. An approach of systemic characterization, classification, and comparison. Chem. Lett., 1991; 1991, 1097–1100. https://doi.org/10.1246/cl.1991.1097

Hamano, K., Takasawa, T., Kurazono, T., Okuyama, Y., Nishigaki, K. Genome profiling—establishment and practical evaluation of its methodology. Nikkashi (Bull. Chem. Soc. Jpn.), 1996; 1996(1): 54–61, ja: . The term 'DNA profiling' was changed to 'genome profiling' to avoid confusion, as the term 'DNA profiling' had begun to be used for a different technology in the field of forensics, as expressed in the article 'The use of DNA profiling and behavioural science in the investigation of sexual offences' by Davies, A., published in Med Sci Law (1991). https://pubmed.ncbi.nlm.nih.gov/2062204/</ref>. In GP, small fragments of genomic DNA are randomly amplified (random PCR) and the random PCR products are subjected to temperature-gradient gel electrophoresis (TGGE) to generate a species-specific mobility pattern (genome profile). From this, species identification dots (spiddos) are assigned<ref>https://doi.org/10.1093/nar/30.10.e42</ref>. This approach is clearly superior because it does not require prior knowledge of any gene sequence. It is clear that random PCR can produce commonly conserved genetic fragments (ccgf), which make it possible to measure the difference between organisms <ref>https://doi.org/10.1093/nar/30.10.e42</ref>. The GP method has been successfully applied to a wide range of organisms, from viruses and bacteria to animals and plants, for identification and classification <ref>https://doi.org/10.1093/bfgp/elae047</ref>. Its unique merit is in the ultra-high performance to obtain the final results (identification and classification), since GP, in principle, requires only a single random PCR plus μTGGE experiment (~2 h task in all)

Procedure and Theory

Procedure and Theory