Pyrethroid hydrolase

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The enzyme '''pyrethroid hydrolase''' (EC 3.1.1.88, '''pyrethroid-hydrolyzing carboxylesterase, pyrethroid-hydrolysing esterase, pyrethroid-hydrolyzing esterase, pyrethroid-selective esterase, pyrethroid-cleaving enzyme, permethrinase, PytH, EstP'''; systematic name '''pyrethroid-ester hydrolase''')<ref>{{cite journal | vauthors = Wang BZ, Guo P, Hang BJ, Li L, He J, Li SP | title = Cloning of a novel pyrethroid-hydrolyzing carboxylesterase gene from ''Sphingobium'' sp. strain JZ-1 and characterization of the gene product | journal = Applied and Environmental Microbiology | volume = 75 | issue = 17 | pages = 5496–500 | date = September 2009 | pmid = 19581484 | pmc = 2737939 | doi = 10.1128/aem.01298-09 }}</ref><ref>{{cite journal | vauthors = Wu PC, Liu YH, Wang ZY, Zhang XY, Li H, Liang WQ, Luo N, Hu JM, Lu JQ, Luan TG, Cao LX | title = Molecular cloning, purification, and biochemical characterization of a novel pyrethroid-hydrolyzing esterase from ''Klebsiella'' sp. strain ZD112 | journal = Journal of Agricultural and Food Chemistry | volume = 54 | issue = 3 | pages = 836–42 | date = February 2006 | pmid = 16448191 | doi = 10.1021/jf052691u }}</ref><ref>{{cite journal | vauthors = Liang WQ, Wang ZY, Li H, Wu PC, Hu JM, Luo N, Cao LX, Liu YH | title = Purification and characterization of a novel pyrethroid hydrolase from ''Aspergillus niger'' ZD11 | journal = Journal of Agricultural and Food Chemistry | volume = 53 | issue = 19 | pages = 7415–20 | date = September 2005 | pmid = 16159167 | doi = 10.1021/jf051460k }}</ref><ref>{{cite journal | vauthors = Stok JE, Huang H, Jones PD, Wheelock CE, Morisseau C, Hammock BD | title = Identification, expression, and purification of a pyrethroid-hydrolyzing carboxylesterase from mouse liver microsomes | journal = The Journal of Biological Chemistry | volume = 279 | issue = 28 | pages = 29863–9 | date = July 2004 | pmid = 15123619 | doi = 10.1074/jbc.m403673200 | doi-access = free }}</ref><ref>{{cite journal | vauthors = Maloney SE, Maule A, Smith AR | title = Purification and preliminary characterization of permethrinase from a pyrethroid-transforming strain of ''Bacillus cereus'' | journal = Applied and Environmental Microbiology | volume = 59 | issue = 7 | pages = 2007–13 | date = July 1993 | pmid = 8357241 | pmc = 182228 }}</ref><ref>{{cite journal | title = Pyrethroid-degrading ''Sphingobium'' sp. JZ-2 and the purification and characterization of a novel pyrethroid hydrolase |author1 = Guo, P. |author2 =Wang, B. |author3 =Hang, B. |author4 =Li, L. |author5 =Ali, W. |author6 =He, J. |author7 =Li, S. | journal = Int. Biodeter. Biodegrad. |year = 2009 |volume = 63 |issue = 8 |pages = 1107–1112 |doi=10.1016/j.ibiod.2009.09.008}}</ref> [[catalysis|catalyses]] the reaction

The enzyme '''pyrethroid hydrolase''' (EC 3.1.1.88, '''pyrethroid-hydrolyzing carboxylesterase, pyrethroid-hydrolysing esterase, pyrethroid-hydrolyzing esterase, pyrethroid-selective esterase, pyrethroid-cleaving enzyme, permethrinase, PytH, EstP'''; systematic name '''pyrethroid-ester hydrolase''')<ref>{{cite journal | vauthors = Wang BZ, Guo P, Hang BJ, Li L, He J, Li SP | title = Cloning of a novel pyrethroid-hydrolyzing carboxylesterase gene from ''Sphingobium'' sp. strain JZ-1 and characterization of the gene product | journal = Applied and Environmental Microbiology | volume = 75 | issue = 17 | pages = 5496–500 | date = September 2009 | pmid = 19581484 | pmc = 2737939 | doi = 10.1128/aem.01298-09 | bibcode = 2009ApEnM..75.5496W }}</ref><ref>{{cite journal | vauthors = Wu PC, Liu YH, Wang ZY, Zhang XY, Li H, Liang WQ, Luo N, Hu JM, Lu JQ, Luan TG, Cao LX | title = Molecular cloning, purification, and biochemical characterization of a novel pyrethroid-hydrolyzing esterase from ''Klebsiella'' sp. strain ZD112 | journal = Journal of Agricultural and Food Chemistry | volume = 54 | issue = 3 | pages = 836–42 | date = February 2006 | pmid = 16448191 | doi = 10.1021/jf052691u | bibcode = 2006JAFC...54..836W }}</ref><ref>{{cite journal | vauthors = Liang WQ, Wang ZY, Li H, Wu PC, Hu JM, Luo N, Cao LX, Liu YH | title = Purification and characterization of a novel pyrethroid hydrolase from ''Aspergillus niger'' ZD11 | journal = Journal of Agricultural and Food Chemistry | volume = 53 | issue = 19 | pages = 7415–20 | date = September 2005 | pmid = 16159167 | doi = 10.1021/jf051460k | bibcode = 2005JAFC...53.7415L }}</ref><ref>{{cite journal | vauthors = Stok JE, Huang H, Jones PD, Wheelock CE, Morisseau C, Hammock BD | title = Identification, expression, and purification of a pyrethroid-hydrolyzing carboxylesterase from mouse liver microsomes | journal = The Journal of Biological Chemistry | volume = 279 | issue = 28 | pages = 29863–9 | date = July 2004 | pmid = 15123619 | doi = 10.1074/jbc.m403673200 | doi-access = free }}</ref><ref>{{cite journal | vauthors = Maloney SE, Maule A, Smith AR | title = Purification and preliminary characterization of permethrinase from a pyrethroid-transforming strain of ''Bacillus cereus'' | journal = Applied and Environmental Microbiology | volume = 59 | issue = 7 | pages = 2007–13 | date = July 1993 | doi = 10.1128/aem.59.7.2007-2013.1993 | pmid = 8357241 | pmc = 182228 | bibcode = 1993ApEnM..59.2007M }}</ref><ref>{{cite journal | title = Pyrethroid-degrading ''Sphingobium'' sp. JZ-2 and the purification and characterization of a novel pyrethroid hydrolase |author1 = Guo, P. |author2 =Wang, B. |author3 =Hang, B. |author4 =Li, L. |author5 =Ali, W. |author6 =He, J. |author7 =Li, S. | journal = Int. Biodeter. Biodegrad. |year = 2009 |volume = 63 |issue = 8 |pages = 1107–1112 |doi=10.1016/j.ibiod.2009.09.008|bibcode = 2009IBiBi..63.1107G }}</ref> [[catalysis|catalyses]] the reaction

: ''trans''-permethrin + H₂O <math>\rightleftharpoons</math> (3-phenoxyphenyl)methanol + (1''S'',3''R'')-3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylate

: ''trans''-permethrin + H₂O <math>\rightleftharpoons</math> (3-phenoxyphenyl)methanol + (1''S'',3''R'')-3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylate