Pimelyl-(acyl-carrier protein) methyl ester esterase

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The enzyme '''Pimelyl-[acyl-carrier protein] methyl ester esterase''' (EC 3.1.1.85, '''BioH'''; systematic name '''pimelyl-[acyl-carrier protein] methyl ester hydrolase'''<ref>{{cite journal | vauthors = Sanishvili R, Yakunin AF, Laskowski RA, Skarina T, Evdokimova E, Doherty-Kirby A, Lajoie GA, Thornton JM, Arrowsmith CH, Savchenko A, Joachimiak A, Edwards AM | title = Integrating structure, bioinformatics, and enzymology to discover function: BioH, a new carboxylesterase from ''Escherichia col''i | journal = The Journal of Biological Chemistry | volume = 278 | issue = 28 | pages = 26039–45 | date = July 2003 | pmid = 12732651 | pmc = 2792009 | doi = 10.1074/jbc.M303867200 | doi-access = free }}</ref><ref>{{cite journal | vauthors = Lemoine Y, Wach A, Jeltsch JM | title = To be free or not: the fate of pimelate in ''Bacillus sphaericus'' and in ''Escherichia coli'' | journal = Molecular Microbiology | volume = 19 | issue = 3 | pages = 645–7 | date = February 1996 | pmid = 8830257 | doi = 10.1046/j.1365-2958.1996.t01-4-442924.x }}</ref><ref>{{cite journal | vauthors = Tomczyk NH, Nettleship JE, Baxter RL, Crichton HJ, Webster SP, Campopiano DJ | title = Purification and characterisation of the BIOH protein from the biotin biosynthetic pathway | journal = FEBS Letters | volume = 513 | issue = 2–3 | pages = 299–304 | date = February 2002 | pmid = 11904168 | doi = 10.1016/s0014-5793(02)02342-6 | doi-access = free }}</ref><ref>{{cite journal | vauthors = Lin S, Hanson RE, Cronan JE | title = Biotin synthesis begins by hijacking the fatty acid synthetic pathway | journal = Nature Chemical Biology | volume = 6 | issue = 9 | pages = 682–8 | date = September 2010 | pmid = 20693992 | pmc = 2925990 | doi = 10.1038/nchembio.420 }}</ref> [[catalysis|catalyses]] the reaction

The enzyme '''Pimelyl-[acyl-carrier protein] methyl ester esterase''' (EC 3.1.1.85, '''BioH'''; systematic name '''pimelyl-[acyl-carrier protein] methyl ester hydrolase'''<ref>{{cite journal | vauthors = Sanishvili R, Yakunin AF, Laskowski RA, Skarina T, Evdokimova E, Doherty-Kirby A, Lajoie GA, Thornton JM, Arrowsmith CH, Savchenko A, Joachimiak A, Edwards AM | title = Integrating structure, bioinformatics, and enzymology to discover function: BioH, a new carboxylesterase from ''Escherichia col''i | journal = The Journal of Biological Chemistry | volume = 278 | issue = 28 | pages = 26039–45 | date = July 2003 | pmid = 12732651 | pmc = 2792009 | doi = 10.1074/jbc.M303867200 | doi-access = free }}</ref><ref>{{cite journal | vauthors = Lemoine Y, Wach A, Jeltsch JM | title = To be free or not: the fate of pimelate in ''Bacillus sphaericus'' and in ''Escherichia coli'' | journal = Molecular Microbiology | volume = 19 | issue = 3 | pages = 645–7 | date = February 1996 | pmid = 8830257 | doi = 10.1046/j.1365-2958.1996.t01-4-442924.x }}</ref><ref>{{cite journal | vauthors = Tomczyk NH, Nettleship JE, Baxter RL, Crichton HJ, Webster SP, Campopiano DJ | title = Purification and characterisation of the BIOH protein from the biotin biosynthetic pathway | journal = FEBS Letters | volume = 513 | issue = 2–3 | pages = 299–304 | date = February 2002 | pmid = 11904168 | doi = 10.1016/s0014-5793(02)02342-6 | doi-access = free | bibcode = 2002FEBSL.513..299T }}</ref><ref>{{cite journal | vauthors = Lin S, Hanson RE, Cronan JE | title = Biotin synthesis begins by hijacking the fatty acid synthetic pathway | journal = Nature Chemical Biology | volume = 6 | issue = 9 | pages = 682–8 | date = September 2010 | pmid = 20693992 | pmc = 2925990 | doi = 10.1038/nchembio.420 }}</ref> [[catalysis|catalyses]] the reaction

: pimelyl-[acyl-carrier protein] methyl ester + H₂O <math>\rightleftharpoons</math> pimelyl-[acyl-carrier protein] + [[methanol]]

: pimelyl-[acyl-carrier protein] methyl ester + H₂O <math>\rightleftharpoons</math> pimelyl-[acyl-carrier protein] + [[methanol]]